Yes... The moment of truth... What actually happened? Heart stroke..? Nah, to cheesy ... OH, decapitation..! Nah, not suitable for the readers... ... Maybe... Death of drug overdose..? ... Ah, I give up, she dies doesn't she?
Hmm. Pinkie Pie's cutie mark has been somewhat reversed: it is normally one yellow balloon in the middle above two blue balloons on the side. And in the last page it two blue balloons above one yellow balloon. I wonder what this foreshadows? Evil changeling clone, or insignificant oversight?
It's somewhat subtle, but after the following events, it simply felt like it was something I had seen in some animes...
1) A villian stalks the main character before anything else. 2) Main character does a last minute Dues Ex Machina (when Fluttershy was defending her... Although to your credit, instead of everything turning into super awesome time, Twilight only manages to barely survive). 3) Angsty teenagers (just kidding)
I remember there being a few more subtle influences but I can't remember them. To be honest, in retrospect, the story becomes WAY MORE dramatic in general and gets less anime-oriented... And don't get me wrong, I didn't mean it in an offensive manner nor that you're ripping off anything, it's just that for most of the beginning strips my brain went "yay anime!" whenever I made such a connection.
The only thing I will say, is this!... You must be getting pretty good at drawing the background for hospitals, huh? xD