Yes... The moment of truth... What actually happened? Heart stroke..? Nah, to cheesy ... OH, decapitation..! Nah, not suitable for the readers... ... Maybe... Death of drug overdose..? ... Ah, I give up, she dies doesn't she?
Hmm. Pinkie Pie's cutie mark has been somewhat reversed: it is normally one yellow balloon in the middle above two blue balloons on the side. And in the last page it two blue balloons above one yellow balloon. I wonder what this foreshadows? Evil changeling clone, or insignificant oversight?
It's somewhat subtle, but after the following events, it simply felt like it was something I had seen in some animes...
1) A villian stalks the main character before anything else. 2) Main character does a last minute Dues Ex Machina (when Fluttershy was defending her... Although to your credit, instead of everything turning into super awesome time, Twilight only manages to barely survive). 3) Angsty teenagers (just kidding)
I remember there being a few more subtle influences but I can't remember them. To be honest, in retrospect, the story becomes WAY MORE dramatic in general and gets less anime-oriented... And don't get me wrong, I didn't mean it in an offensive manner nor that you're ripping off anything, it's just that for most of the beginning strips my brain went "yay anime!" whenever I made such a connection.
The only thing I will say, is this!... You must be getting pretty good at drawing the background for hospitals, huh? xD
1.) A lot of stories, anime and otherwise, have villains stalking the main characters. It's a common plot element, especially when there's past history and purpose for the stalking (and, rest assured, he has a reason.)
2.) Deus Ex Machina usually implies a forced and unnatural intervention. Twilight was right outside of Fluttershy's house, so it's not like her appearance should have been too odd. Unexpected, maybe, but not contrived. I try to avoid irrational plot devices, and try to make sure everything serves a purpose, even if it isn't immediatly seen (a lot of what I do and have done was to set up something later on as opposed to set up something immediatly.)
The only actual influences I draw from anime are the "final form" joke (and even then I did that cause it's a meme). I'm, by my own confession, not a big anime fan - I like Naruto, Bleach, and Fullmetal Alchemist, but that's about the extent of my dip in the anime pool.
Now that I think about... I have probably watched much more anime than any other type of visual media. Weird, I don't feel that way, but now that I remember, I think that I tended to see a lesser variety of anime than other shows (like American cartoons). But in retrospect, I think I've put in more hours watching anime, only because of their tendency to run for so long.
Well, so be it... Btw, I recommend just one more anime that you should at least give a chance: Death Note. It's much shorter than the ones you've listed, with only 37 episodes in total, but I found it very enjoyable.
They have a Ambulance pony? What? I've never heard of such a thing!! Nice work on the emotion in the faces. In the first panel the pink pony flying forward is a nice descriptive gesture for how fast ambulance pony is going. Looks good!